Method of preparing concentrated vitamine b



Patented 15, 1927;

UNITED STATES l 41,617Jl02 PA-TENT OFFICE.

P GACCIA, OF NEW YORK, N. Y.

IE'IEOD OI PREPARIHG CONCENTRATED VITAMINE B.

No Drawing. Original application filed August 7, 1925, Serial no. 48,865. Divided and this application and September 25, 1925. Serial No. 58,607.

The object of my invention is the production of vitamine B in a concentrated state and as distinguished from the present methods, my product, when purified and crystallined, preserves its curative efl'ect in the organism' with more intensity than the commercial vitamines in a mixture of different products, either in dry form or in solution.

In carrying out my invention one pound 'of dry yeastis diluted with one gallon of watch The mixture is placed in a cool place and allowed to stand for several days, the

7 same being stirred from time to time. 1

"then filter the mixture numbering the filtrate 1 and the residue .2. The residue is then triturate'd in a mortar with sand and this nuxture is diluted with av solution of 0.2% H01 andthe-ratio being one liter per 100 grs. of residue. This mixture is allowed.

to stand for two hours, stirring from time to time, after which it is brought slowly to the boilmg point and filtered. The lasti named filtrate is mixed with filtrate 1 and concentrated to form-asticky paste of moisture content; The vitamine B is then extracted from this hygroscopic pasteiprod uct withwarmabsolute alcohol in continued extraction to make the alcoholic solution about 95%, Then I precipitatethe vitamine B fromthis alcoholic extract by the addition an of driedn'ethylene dichloride,which takes up some of the water of said aldoholic extract to make it about 97%-98% in strength the vitamine B being insoluble in this latter strength will precipitate leaving all theimpurities in the alcohol and ethylene dichloride solution. The above precipitate is now dissolved with a Very\small quantity of water, whereupon concentrated sulphuric acid is. added, then warmed gently and al- 40 lowed to stand for some time until the crystals are formed. F 111a y I filter, wash the crystals Withdilutesulphuric acid and then w th ether.

I claim:

A method of deriving concentrated vitamin'e B consisting ina continued extraction of a sticky paste, containing vitamine B, with absolute alcohol, thereby reducing the alcohol to approximately by the 5 PIO 

